LABORATORY RECORD

Name of the Laboratory:

Coordination and Bioinorganic Chemistry Research Laboratory

Unit/Unit(s) Reported to:

Engineeering Faculty

Chemical enginering  and Applied Chemistry

 

Sponsor Information:

ATÜ-BAP and COST projects

LABORATORY CONTACT INFORMATION

Address:

Atılım University, Chemical Engineering and Applied Chemistry, A2 Buildig, Floor -3,  06836 İncek, Ankara

WEB Address:

cobirl.atilim.edu.tr (under construction)

Telephone Number:

+ 90 312 586 8528

Lab Director/Supervisor:

Prof. Dr. Şeniz Özalp-Yaman

seniz.ozalp@atilim.edu.tr

+ 90 312 586 83 36

Lab Personnel:

 

Name Surname

Position

E-mail

     
     

MISSION OF THE LABORATORY

Mission of the Lab:

Education

Undergradute  ¨     Master’s  √     Doctorate √

Research

Undergraduate ¨      Master’s  √     Doctorate √

Service

¨

Undergradute ¨      Master’s  ¨     Doctorate ¨

ABOUT THE LABORATORY

Founding date:

 

Laboratory Area

 30 m2

 

Specialty Area(s) of the Lab:

  • Coordination Chemistry: Synthesis, electronic structure and electronic absorption spectrum of metal complexes
  • Bioinorganic Chemistry: Design and synthesis of drugs especially antitumor drugs, Interactions between DNA-metal ions and DNA-metal complexes
  • Electrochemistry: Electrochemical behavior of metal complexes
  • Bioelectrochemistry: Investigation of binding ability of the complexes on DNA by electrochemical methods
  • Spectroelectrochemistry: Combination of the spectroscopic and electrochemical methods.
  • Inorganic Polymers: Polymers of some Schiff base containing metals

Competencies of the Lab:

 

Name

Function

Bruker-Equinox 55 FTIR/ATR

Used for analysis of the coordination compounds.

Agilent 8453 UV-vis spectrophotometer

Required for UV-Vis analysis, spectroelectrochemical investigations and UV-titrations of the complexes with DNA.

Radiometer Analytical VoltaLab PGZ301 Potentiostat

Used for the electrochemical investigations of the coordination compounds and dna-metal complex interactions or protein-metal complex interactions.

Water Bath (Haake)

Used for the incubation of protein or DNA solutions with the metal complexes.

Criostat (Haake)

Used in the constant temperature and low temperature experiments.

Electrophoresis Systems

Used in DNA-metal complex interaction experiments

Ultrasonic bath  

Used to prepare homogeneous solutions.

Bench top centrifuge

Used for seperation purposes.

Vortex

Used to obtain homogeneous mixtres.

Sonicator

Used to obtain homogeneous mixtres.

Gel Monitoring sysytem

Used to monitor the gels after the electrophoresis

Ultra Pure water system (Millipore)

Required for all DNA ve the biological experiments

Vacuum system (Büchi V-1000)               

Used for drying and crystalliation.

Vacuum Oven (Fisher ISOTEMP-282 A)

Used for drying purpose .

 

Completed Projects till today/On-going Projects:

 

Project Name

Relevant Institution/ Organization

Budget

Beginning Ending date  

Synthesis, Electrochemistry, and Antitumor Activities of  New Platinum Blue Complexes

BAP/ ATÜ

 

1.7.2006 - 30.6.2008

Summary (Summary, findings, results, outcomes 100 Words)

A family of deeply colored platinum compounds, usually called platinum blues, has attracted wide interest for years not only because of their unusual color and interesting chemistry, but also for their high antitumor activities On the bases of the results gathered from elemental analysis, UV-Vis, ESR, XPS, 1H-, 13C- and 195Pt-NMR, CV measurements it is proposed that the blue complex is a new “platinum blues”, first time synthesized in this work, with a formula [Pt4(2-atp)8(OH)(H2O)]. The structure can be visualized as the dimer of the head-to-head isomer of binuclear [Pt2(2-atp)4] complex. It is a mixed valence complex with one platinum “+3” and the other three platinums “+2” oxidation state in tetranuclear unit “Pt(III) Pt(II)3”. All platinum blues reported in the literature so far contains nitrogen and oxygen donor atom ligands. The blue complex prepared in this work is the first example of the platinum blues, which contain nitrogen and sulfur donor ligands. It is also interesting that in all previous platinum blues the bridging ligand forms 5-membered ring upon coordination (two platinum atoms and three ligand atoms), in our case the bridging ligand forms 6-membered ring upon coordination. Decrease in the electronic absorption band of the complex upon addition of DNA solution with a slight red shift, indicates the intercalative binding of the blue complex between the base stacts of DNA. Gel electrophoresis results showed thwt the complex perpofm double strand scission in 25 nM. Enzymatic activity of the blue complex was determined in sheep liver GST enzymes  spectroscopically. The blue complex inhibits the GSTs activity between 600 -7200 nM, indicating that the complex can be uesd as a codrug during the chemotheraphy.

Project Name

Relevant Institution/ Organization

Budget

Beginning Ending Date

Synthesis, Electrochemical Characterızation and DNA Binding Activity of New Platinum(II) Complexes Containing Oxime Derivative Ligands

BAP/ ATÜ

 

15.02.2010 - 15.6.2012

Summary

The main aim of this study is to synthesize novel platinum complexes with variety of ligands of oximes as potential anticancer drug leads providing better efficacy with low tissue resistance compared to cisplatin.  The change in the DNA (Deoxyribonucleic acid: gene) conformation that is generated by the interaction of the complexes will be determined by electrophoresis with some spectroscopic and electrochemical methods. Electrochemical investigations of the platinum complexes containing pyridine (L1) and thiophen (L3) oximes, indicates that, electron is removed from an metal based molecular orbital by producing Pt(III) and then Pt(IV)complexes, consecutively. On the other hand, the platinum complexes containing pyrole oxime (L2) is oxidized in one step through the metal center, generating Pt(IV)-complexes. Interaction of the complexes on DNA is studied by the elecrochemical methods and teh diffussion contansts (Df, Db), the binding constants (Kb) and the binding site size (s) values are determined for all the complexes. According to the binding constantans, the activity order of oxime complexes can be given as follows; Cis 2 > Bis 2> Cis 3> Bis 1 ≥ Cis 1 > Asy 1 > Bis 3.

Project Name

Relevant Institution/ Organization

Budget

Beginning Ending Date

Synthesis Electrochemical Characterization and antioxidative anticancer effects of new platinum(II) complexes containing oxime derivative ligands

COST (TÜBİTAK)

 

15.02.2010 - 15.6.2012

Summary

 

Antitumor activity of the oxime groups is well known. Therefore, 9 novel platinum complexes with three different class of oximes ligands are prepared inorder to increase the antitumor activity of the oximes with a synergic effect. The UV titration studies reveal that complexes are intercalete between the base stacks of the DNA. The activity order of the complexes can be given as  Cis 2 > Bis 2> Cis 3> Bis 1 ≥ Cis 1 > Asy 1 > Bis 3, according to their binding contants. Gel electrophores studies indicate that all Bis complexes are very active compared to the other complexes in the absence of a reductant. Among the Bis complexes, Bis 2 cleaves the DNA’s double strand hydrolytically in 50 nM. On the other hand, all the ligands, Cis1 and Cis 3 are very active than the other complexes in the presence of a reductant. Cis 1 is the most active complex and cleave the single stand of DNA in 10 nM, oxidatively. Additionally,  the effect of the Pt(II) complexes on the GST, GPX, KAT, PTK ve SOD enzyme activities is studied and Bis 3 is found as an important metal complex due to its higher activity on GST enzyme and antioxidative effect.

Project Name

Relevant Institution/ Organization

Budget

Beginning Ending Date

Yeni Bimetalik Antitümor İlaçlarının DNA ile Etkileşimlerinin Elektrokimyasal Yöntemlerle Belirlenmesi

BAP/ ATÜ

 

Sözleşmesi henüz imzalanmadı. Muhtemel başlangıç tarihi Temmuz 2013

Summary

Among the new strategies which find application in cancer chemotheraphy, the treatment of the binuclear complexes has proven drug’s efficiency on tumors. Therefore, the design and synthesis of new platinum drugs containing pyridinyl ve tiyophenyl ligands have been prompted in order to reduce severe side effects, to overcome drug resistance and to improve the patient’s quality of life.

The main aim of this study is to synthesize novel bimetallic complexes as potential anticancer drug leads providing better efficacy with low tissue resistance compared to cisplatin. Electrochemical behavior of the complexes is studied by cyclic voltammetry (CV) and the constant potential electrolysis of the complexes is carried out at the peak potentials. The change in the electronic absorption spectra of the complexes is followed in situ by UV-Vis spectrophotometer during the electrolysis in order to identify the electrolysis products and possible reaction intermediates. Any possible structural deformations on the DNA-double helix by the interaction of the new bimetallic complexes are determined by electrophoresis.